Study finds common chemicals may alter normal breast development

by Genevieve Rajewski, Tufts University

edited by Sadie Harley, reviewed by Alexander Pol

Sadie Harley

Scientific Editor

Meet our editorial team
Behind our editorial process

Alexander Pol

Deputy Editor

Meet our editorial team
Behind our editorial process Editors' notes

This article has been reviewed according to Science X's editorial process and policies. Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

proofread

The GIST Add to Preferred Sources

Exposure to common chemicals may alter how normal human breast tissue develops, according to a new study from researchers at Tufts University School of Medicine and Boston University. The findings, published in the Proceedings of the National Academy of Sciences, suggest that exposure to some chemicals—particularly multiple bisphenols at once—may create molecular changes in breast tissue that could influence susceptibility to breast disease later in life.

The findings lay the groundwork for scientists to investigate whether these molecular changes persist in human tissues, contribute to disease later in life and could yield biomarkers to help assess breast cancer risk.

"If these results are validated by additional studies, they could change how we think about cancer prevention," said Charlotte Kuperwasser, the study's senior author and a professor of developmental, molecular and chemical biology at Tufts University School of Medicine.

A growing body of research has raised questions about whether exposure to endocrine-disrupting chemicals—substances that can interfere with the body's hormones—may contribute to breast cancer risk. But much of what scientists know about how chemicals affect breast tissue comes from animal models or laboratory-grown cells that cannot fully reproduce the complexity of human tissue.

"The breast is also unusual in that it undergoes major developmental changes well after birth, including during puberty and pregnancy," said Kuperwasser. "And the concern is that breast tissue cells may be highly sensitive to environmental cues, such as chemical exposure, during critical windows of mammary gland development."

Modeling chemical exposure in human tissue

To better capture this complexity, the researchers used three-dimensional models called organoids, grown from breast tissue donated by 13 healthy young women. The organoids contain multiple types of breast cells and can reproduce some aspects of how human breast tissue actually develops.

As the organoids developed over about three weeks, the researchers exposed them to a panel of individual chemicals and mixtures, including endocrine-disrupting chemicals, chemicals that affect metabolism, and known or suspected carcinogens. They then analyzed molecular changes, including changes in gene activity and DNA modifications.

Bisphenol mixtures disrupt normal development

While different chemicals affected tissue development in different ways, the most striking finding involved three common bisphenols: BPA, BPS and BPF. Individually, the chemicals produced relatively few changes in the organoids. But when combined at very low concentrations—comparable to levels detected in human blood and urine—the mixture disrupted normal breast development in organoids from most of the donors.

"We intentionally used concentrations of chemicals that were relevant to real-world exposure rather than the very high doses sometimes used in toxicology studies," said Kuperwasser. "We also tested a mixture of these because people are typically exposed to multiple chemicals at the same time."

The team then compared the molecular changes associated with the bisphenol mixture with molecular data from human breast cancers. The exposure-associated pattern was most strongly represented in data from patients with invasive lobular carcinoma—a type of breast cancer that may not cause a detectable lump and can be difficult to detect with a mammogram.

Molecular changes are not proof of cancer

The finding does not establish that the chemicals cause cancer or show how the observed molecular changes might contribute to breast disease. Instead, the researchers say the research demonstrates a new way to study how environmental exposures may affect normal human tissue before cancer develops.

"Historically, we have thought about cancer risk largely in terms of inherited mutations, lifestyle factors and exposures that directly damage DNA," said Kuperwasser. "These findings raise another possibility: Environmental exposures may subtly alter how normal tissues develop, leaving behind molecular changes that do not cause cancer by themselves but may make the tissue more permissive to cancer many years later."

She noted that such persistent molecular changes could eventually serve as a kind of biological record—like a "scar" of past exposure. If validated in people, such a marker could potentially help identify individuals at higher risk and inform earlier or more targeted screening.

Future studies involving larger and more diverse groups of donors, as well as a broader range of chemicals and mixtures, are needed to determine how widely the findings apply and if molecular changes observed in organoids are associated with breast disease in people.

Meadow Parrish and Meloryn Seraj, both Ph.D. students in genetics, molecular and cellular biology at Tufts Graduate School of Biomedical Sciences, are co-first authors on the study.

Publication details

Meadow Parrish et al., Environmental chemical mixtures reprogram mammary epithelial development to epigenetic states associated with breast cancer, Proceedings of the National Academy of Sciences (2026). DOI: 10.1073/pnas.2613054123

Journal information: Proceedings of the National Academy of Sciences

Key medical concepts

Organoids

Clinical categories

OncologyWomen's healthPreventive medicineCommon illnesses & Prevention Provided by Tufts University Who's behind this story?

Sadie Harley

BSc Life Sciences & Ecology. Microbiology lab background with pharmaceutical news experience in oil, gas, and renewable industries. Full profile →

Alexander Pol

PhD nano-engineering from Delft University. Published researcher and journal reviewer. Brings scientific insight to content standards. Full profile →

Citation: Study finds common chemicals may alter normal breast development (2026, October 16) retrieved 16 October 2026 from https://medicalxpress.com/news/2026-10-common-chemicals-breast.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.