Children's brain tumors may spread faster when microglia build invasion-friendly scaffolding

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by Anna Persson, Karolinska Institutet

edited by Lisa Lock, reviewed by Robert Egan

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Credit: Karolinska Institutet

Researchers at the Institute of Environmental Medicine (IMM), Karolinska Institutet, have identified a possible mechanism behind the spread of the aggressive brain tumor diffuse midline glioma. The study shows that the brain's own immune cells, microglia, may contribute to the tumor's invasive capacity by producing the protein fibronectin. The results are published in the journal Cell Death & Disease.

Diffuse midline glioma (DMG), also known as diffuse intrinsic pontine glioma (DIPG), is a rare but highly aggressive brain tumor that primarily affects children. There is currently no effective treatment, and the prognosis is very poor.

In the present study, the researchers investigated how microglia—the brain's immune cells—are affected by tumor cells and what role they play in disease progression.

The researchers observed that microglia exposed to DMG cells began producing large amounts of extracellular matrix proteins, particularly fibronectin. Analyses of patient samples and single‑cell data also showed that microglia were the main source of fibronectin in the tumors.

"Our results suggest that microglia not only react to the tumor but actively contribute to making it more invasive," says Professor Bertrand Joseph at IMM.

In laboratory experiments, the researchers demonstrated that fibronectin from microglia increased the tumor cells' ability to invade surrounding tissue. When fibronectin was blocked—chemically or genetically—the invasiveness of the tumor cells was clearly reduced.

The study also shows that high levels of fibronectin and other matrix proteins were associated with poorer prognosis in several independent patient cohorts.

"Fibronectin emerges as a potential therapeutic target in a disease where treatment options are currently very limited," says Bertrand Joseph.

The researchers hope that, in the long term, the results may contribute to the development of new treatment strategies for DMG.

More information

Lily Keane et al, Microglia in diffuse midline glioma contribute to extracellular matrix remodelling and cancer cell invasion, Cell Death & Disease (2026). DOI: 10.1038/s41419-026-08891-y

Key medical concepts

FibronectinMicroglia

Clinical categories

OncologyChildren's healthPediatricsNeurology Provided by Karolinska Institutet Who's behind this story?

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