New mechanism reveals how atypical E. coli maintain their ability to infect

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by Hebrew University of Jerusalem

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The structures used by the bacteria to attach to the human cells: the left image shows regular-shaped bacteria with many hair-like structures (green) projecting from their surface. The right image shows these structures can cover the entire length of the filamentous bacteria. The white lines at the bottom represent microscopic scale of 2 micrometer = 1/500 of a millimeter). Credit: Noam Yedidi, Ilan Rosenshine's lab

A new study shows how a type of disease-causing E. coli can quickly adapt when it loses one of its main ways of attaching to the intestine. The bacteria either change shape or make small changes to a protein that strengthens their grip on human cells. The findings help explain why these strains can still cause illness and identify a possible target for future treatments that could stop infection by preventing bacteria from attaching in the first place.

For a bacterium trying to cause an infection, the first challenge is simple: don't get swept away.

Inside the human intestine, disease-causing E. coli must latch onto the cells lining the gut. If it cannot hold on, it is much less likely to establish an infection. But a new study shows that when some E. coli bacteria lose one of their main attachment tools, they can rapidly find another way to cling to human cells and make that new grip much stronger.

The research, led by Noam Yedidi, a Ph.D. student, and professors Ilan Rosenshine and Sigal Ben-Yehuda of the Institute for Medical Research Israel-Canada (IMRIC) at the Hebrew University of Jerusalem, was published in Gut Microbes.

The team studied enteropathogenic Escherichia coli, or EPEC, a type of E. coli that can cause severe diarrhea, especially in young children. Persistent infection can be particularly harmful to children, affecting both physical growth and development.

How to direct evolution in the lab: e. coli bacteria lacking their major attachment capabilities were incubated with human cells in a petri dish. After infection, the plate was washed repeatedly to remove the floating bacteria, and the few bacteria that were able to attach to the human cells remained in the plate. These few bacteria were collected and grown, then used for another cycle of infection. Four successive cycles of selection generated bacteria with highly efficient attachment. Credit: Noam Yedidi, Ilan Rosenshine's lab

Many traditional EPEC strains use tiny hairlike structures to attach to cells in the intestine. Yet in recent years, "atypical" EPEC strains, bacteria that lack this important attachment system, have become increasingly common.

That raised an important question: If these bacteria have lost one of their main ways to hold onto the gut, how are they still causing infections?

To find out, the researchers recreated this challenge in the laboratory. They started with bacteria that lacked their major attachment mechanisms, then repeatedly selected the few bacteria that still managed to stick to human cells.

The result was striking. After only four rounds of selection, the bacteria had evolved a far stronger ability to attach.

They did so in two ways. Some bacteria became unusually long, creating more surface area and allowing many small attachment structures to work together (image 3). Others developed small genetic changes in a protein called FimH, found at the tip of these structures. Those changes made the protein bind more tightly to molecules on human cells.

In some cases, the evolved bacteria attached to human cells more than 100 times better than the original strain.

"It is like losing a hook and quickly learning to use another one," said Rosenshine. "The bacteria do not need to invent an entirely new system. They can take a tool they already have and improve it."

The researchers then asked whether the same process might be happening outside the laboratory. They examined the genomes of 327 atypical EPEC strains isolated from patients and found changes in the fimH gene in about half of them. When the researchers recreated and tested many of these naturally occurring changes, they found that several made the bacteria attach to human cells 10 to 100 times more strongly.

The bacteria's improved grip also appeared to make infection more effective. EPEC uses a needle-like molecular system to inject proteins into human cells and interfere with their normal functions. Bacteria with stronger attachment were better able to deliver these proteins.

The findings do not yet offer a treatment, but they point to a possible future strategy. Rather than trying only to kill the bacteria, scientists may be able to interfere with their ability to attach to the intestine in the first place. Drugs that block FimH are already being studied for other E. coli infections, although more research is needed before this approach could be considered for atypical EPEC.

More broadly, the study offers a vivid example of evolution in action: When bacteria lose an important tool, they may not become weaker. Sometimes, they find another tool—and quickly learn to use it better.

More information

Noam Yedidi et al, Parallel evolutionary trajectories rewire enteropathogenic Escherichia coli adhesion to restore host attachment, Gut Microbes (2026). DOI: 10.1080/19490976.2026.2725431

Clinical categories

Infectious diseasesGastroenterologyCommon illnesses & Prevention Provided by Hebrew University of Jerusalem Who's behind this story?

Gaby Clark

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