Innovative annatto-based therapy shows promise for treating pediatric halitosis

The researchers selected 52 mouth-breathing children aged 6-12 with a diagnosis of halitosis confirmed using an inexpensive halimeter available from online retailers (enabling dentists to perform the diagnostic protocol easily in their consulting rooms).

The first author of the article is Laura Hermida Cardoso, a PhD candidate under the Interinstitutional Doctorate Program (Dinter) run by Nove de Julho University (UNINOVE) in São Paulo (Brazil) and the Catholic University of Uruguay (UCU) in Montevideo. The co-authors are all researchers at the Metropolitan University of Santos (UNIMES) in São Paulo state.

Antimicrobial photodynamic therapy (aPDT) consists of the administration of a photosensitizer combined with a light source at a specific wavelength, and oxygen. The procedure generates reactive oxygen species that induce bacterial cell death. Although the study only involved children, the method can treat halitosis in people of any age.

Bussadori, who is also a full professor at UNIMES and chairs the Brazilian Pediatric Dentistry Association (ABOPED), recalled that she has observed many cases of halitosis when treating children and adolescents. "These observations led me to study halitosis in depth with the aim of developing simple and effective treatment protocols," she said.

Affordability

According to Bussadori, any reference to photodynamic therapy in an academic context brings to mind as first option the usual method that involves methylene blue photosensitizer and red laser light.

After several formulations, in vitro experiments and clinical trials, Bussadori succeeded in perfecting an annatto spray, which she patented in 2020. Annatto pods and seeds are dark red when ripe.

The 52 children that met the criteria for inclusion in the study were given instructions on toothbrushing with fluoridated toothpaste and dental flossing three times per day after meals for 30 days.

They were randomly divided into two groups. One was given aPDT applied to the middle third of the dorsal surface of the tongue. The other used a tongue scraper and did not receive aPDT. Both groups used toothbrushing and flossing as noted.

Whitish or yellowish tongue coating is made up mainly of bacteria, metabolites and food debris that usually accumulate on the posterior portion of the tongue dorsum. Several studies have demonstrated that there is a correlation between tongue coating and excessive concentrations of volatile sulfur compounds produced by bacteria, leading to bad breath, but the study led by Bussadori did not find a direct correlation between tongue coating and halitosis in mouth-breathing children. "The main cause of bad breath in these children appears to be oral dryness due to mouth breathing," she said.

Halitosis improved significantly in both groups, but more so in the group that received aPDT.

Halitosis can result in social problems, impair quality of life and indicate more serious medical conditions. In children, it can have a negative influence on psychological development in a critical period of social interaction.

Source:

Fundação de Amparo à Pesquisa do Estado de São Paulo

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