Enhanced TnpB protein offers new possibilities for gene editing

CRISPR-Cas is used broadly in research and medicine to edit, insert, delete or regulate genes in organisms. TnpB is an ancestor of this well-known "gene scissor" but is much smaller and thus easier to transport into cells. Using protein engineering and AI algorithms, UZH researchers have now enhanced TnpB capabilities to make DNA editing more efficient and versatile, paving the way for treating a genetic defect for high cholesterol in the future.

CRISPR-Cas systems, which consist of protein and RNA components, originally developed as a natural defense mechanism of bacteria to fend off intruding viruses. Over the last decade, re-engineering these so-called "gene scissors" has revolutionized genetic engineering in science and medicine. The tools can be programmed to find a specific location in our DNA and edit the genetic information in a precise manner. For example, a disease-causing mutation in the DNA can be reverted to its healthy state.

Much smaller genome editing tool

It was recently discovered that Cas proteins evolved from much smaller proteins, with TnpB being the progenitor of Cas12. Since the large size of Cas proteins creates challenges when trying to deliver them to the right cells in the body, recent studies tried to use their smaller evolutionary progenitors as a genome editing tool. The problem with these small alternatives is that they function less efficiently. This hurdle has now been tackled by a research team headed by Gerald Schwank from the Institute of Pharmacology and Toxicology at the University of Zurich (UZH) together with colleagues from the ETH Zurich.

Gerald Schwank, Institute of Pharmacology and Toxicology, University of ZurichBy engineering the small but powerful protein TnpB, we were able to design a variant that shows a 4.4-fold increase in efficiency of modifying DNA - making it more effective as a gene editing tool."

TnpB proteins are found in a variety of bacteria and archaea. The TnpB studied by the researchers comes from the bacterium Deinococcus radiodurans. This microbe survives cold, dehydration, vacuum and acid, and is one of the most radiation-resistant organisms known to humans. The compact TnpB protein has previously been shown to work for genome editing in human cells, albeit with low efficiency and limited targeting ability due to its recognition requirements when binding DNA.

Better binding ability and broader range of DNA target sequences

Therefore, the researchers optimized TnpB so that it edits the DNA of mammalian cells more efficiently than the original protein. "The trick was to modify the tool in two ways: first, so that it more efficiently goes to the nucleus where the genomic DNA is located, and second, so that it also targets alternative genome sequences," says Kim Marquart, PhD student in Gerald Schwank's lab and first author of the study.

To identify which features in the DNA sequences of the target sites determine the genome editing efficiency, the researchers tested TnpB at 10,211 different target sites. In collaboration with the team of Michael Krauthammer, also professor at UZH, they developed a new artificial intelligence model capable of predicting TnpB editing efficiencies at any given target site. "Our model can predict how well TnpB will work in different scenarios, making it easier and faster to design successful gene editing experiments. Using these predictions, we achieved up to 75.3% efficiency in mouse livers and 65.9% in mouse brains," Marquart adds.

Gene editing therapy of genetic defect for high cholesterol

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University of Zurich

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